Due to increased interest in peptide drugs, Peptide Libraries are in high demand.  The Peptide Library is widely used in drug screening, target validation, T-cell and B-cell epitope mapping, cytotoxic T-cell assays, protein-peptide binding assay, peptide vaccine development, and structure-activity studies. Due to Pi Proteomics, LLC high-throughput parallel peptide synthesis platform, we offer two types of cost-effective Peptide Libraries 1) Crude Peptide Library 2) PurPep Library. This e-mail address is being protected from spam bots, you need JavaScript enabled to view it your peptide sequences along with the type of library you wish built and we will send you a quote back promptly.   

Crude Peptide Library 

Peptide Scale: 2.5 μmol; 1-4mg (3.0 mg average for 12mer peptide)
Peptide Purity: Crude (as synthesized)
Peptide Length :  6-18 amino acids
Peptide Packaging: Lyophilized in individually labeled screw cap vials
Quality Control: MS on every peptide and RP-HPLC on two (2) peptides
Delivery Time: 1-2 weeks
Minimum Order Size: 48 peptides
Guarantee: No guarantee to the purity or success of any one peptide
Optional Modifications: Biotin, Fluorescence, and D-amino acid etc.
Cost:  $29.95 per peptide (price excludes any peptide modifications)
*all peptides with a c-terminal Proline must be amidated, no exceptions.

As the peptides in a Crude Peptide Library are not purified, they cost significantly less than the price of individually made purified peptides. However, by using our optimized synthetic protocols, the peptides in the library typically have purities between 50% - 80%, although this cannot be guaranteed. This purity is sufficient for most initial screening applications. Once a target peptide(s) is identified using the Crude Peptide Library,  it can be remade in high purity and scale using Pi Proteomics’ routine custom peptide synthesis service, for more detailed biological investigations. 

PurPep^TM Library

Peptide Scale: 2.5 μmol; 1-4mg (3.0 mg average for 12mer peptide)
Peptide Purity: ≥70%
Peptide Length:  6-18 amino acids
Peptide Packaging: Lyophilized in individually labeled screw cap vials
Quality Control: CoA, MS and RP-HPLC on every peptide
Delivery Time: 2-3 weeks
Minimum Order Size: 48 peptides
Guarantee: Pi Proteomics Quality Guarantee
Optional Modifications: Biotin, Fluorescence, and D-amino acid
Cost:  $66.95 per peptide (price excludes any peptide modifications)
*all peptides with a c-terminal Proline must be amidated

As the peptides in the PurPep^TM Peptide Library are purified via a chromatography column, they still cost significantly less than the price of individually made purified peptides. However, by using our optimized synthetic protocols and our propriety purification technology the peptides in the library typically have purities between 70% - 90%.  All peptides will be guaranteed to be ≥70% by RP-HPLC and MS. This purity is more than sufficient for most initial screening applications. Once a target peptide(s) is identified using the PurPep^TM Peptide Library,  it can be remade in high purity and scale using Pi Proteomics’ routine custom peptide synthesis service, for more detailed biological investigations.

Here are a few options in the design of the Peptide Library.  If you need assistance, please feel free to This e-mail address is being protected from spam bots, you need JavaScript enabled to view it us and we will be more than willing to assist you in your library design.

Overlapping Peptide Library
Alanine Scanning Library
Truncation Peptide Library
Positional Scanning Library
Scrambled Peptide Library

Overlapping Peptide Library:

Overlapping Peptide Library is ideal for T-cell epitope searching, because T cell epitopes are by nature short linear peptides from the primary protein sequence. The Overlapping Peptide Library is also appropriate for scanning the primary sequence of proteins for linear, or “continuous”, B-cell (antibody-defined) epitopes.
 The overlapping peptide library generation process is defined by two parameters 1) peptide length 2) offset number or degree of overlapping. As a general guideline, shorter peptides are easier to synthesize but they have less chances for multiple epitope hits. More sequence overlapping (small offset number) gives more chances of multiple epitope hits. The offset number reflects the degree of overlapping. Careful selections of these two parameters are important to achieve optimum balance between data value and the experiment cost. Both stated examples increase the number of peptides to be synthesized for the peptide library.

               Overlapping

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Alanine Scanning Library:

Alanine Scanning Library is used to identify specific amino acid residues responsible for a peptide’s activity. Alanine is used to substitute each residue sequentially. Substitution of an essential amino acid results in a reduction in peptide activity, with the degree of activity reduction taken as a relative measure of the importance of the amino acid being substituted.

       Alanine Scanning

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Truncation Peptide Library :

Truncation Peptide Library is used to identify the shortest amino acid sequence needed for biological activity. The Truncation Peptide Library is constructed by systemically removing the flanking residues of the original peptide. If the essential amino acids are known, via the Alanine Scanning, the direction of truncation can be tailored to maintain these residues, while truncating the opposite end.

                  Truncation

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Positional Scanning Library:

Positional Scanning Library is an important tool for sequence optimization. Sequence optimization is achieved by substituting amino acid of interest by all other natural amino acids one at a time.  The Positional Scanning Library has the capability to locate more favorable residue(s) at specified position(s) for enhanced peptide activity.

Positional Scanning

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Scrambled Peptide Library:

Scramble Peptide Library is constructed by carrying out permutation on the original peptide’s sequence. It has the potential to give all possible alternatives and represents the highest level of variability for a peptide library.

Scrambled Peptide Libraries are typically used as:

1. Negative controls (showing specific sequence rather than the amino acid composition is critical for activity)

2. Tool for finding new leads for creating a Positional Scanning Library

                 Scrambled

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